5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, >=99% (HPLC)

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, >=99% (HPLC)

Stock Code: 3578200
Manufacturer Part No: B8503-500MG
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Application


5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.


For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.


Biochem/physiol Actions


5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity. It can be used as a substrate to monitor secreted embryonic alkaline phosphataseactivity in solution.


General description


5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.


Legal Information


BCIP is a registered trademark of Sigma-Aldrich Co. LLC


Packaging


Bottomless glass bottle. Contents are inside inserted fused cone.


Substrates


Histochemical substrate for alkaline phosphatase.

Quality Level200
InChI keyQEIFSLUFHRCVQL-UHFFFAOYSA-N
InChI1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
SolubilityDMF: 20 mg/mL, clear to slightly hazy, yellow
Formpowder
Assay≥99% (HPLC)

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