5-Bromo-4-chloro-3-indolyl phosphate disodium salt, >=98% (HPLC)

Stock Code: 3578182
Manufacturer Part No: B6149-25MG

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Descriptions

Application

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Packaging

1, 5 g in poly bottle

500 mg in poly tube

25, 50, 100 mg in poly bottle

Substrates

Histochemical substrate for alkaline phosphatase.

Specifications

Quality Level	200
InChI key	OAZUOCJOEUNDEK-UHFFFAOYSA-L
InChI	1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
Manufacturer	SIGMA-ALDRICH
Storage Temp.	−20°C
Solubility	DMF: insoluble, H2O: 20 mg/mL
Form	powder
Assay	≥98% (HPLC)