4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate

Stock Code: 3578555
Manufacturer Part No: N3254-100G
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Application


4-Nitrophenyl phosphate di(tris) salt has been used:as a substrate to quantify tartrate-resistant acid phosphatase (TRAP) activity in cell lysatesto determine phosphatase activity using native polyacrylamide gel electrophoresis (PAGE)for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA)


Biochem/physiol Actions


p-Nitrophenyl Phosphate (pNPP) is a preferred substrate for alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures, because of its high sensitivity. This substrate produces a soluble yellow end-product and can be read spectrophotometrically at 405 nm.

Quality Level100
InChI keyXXAXKCWOTRABOW-UHFFFAOYSA-N
InChI1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitywater: 50 mg/mL, clear, colorless to light yellow
Formpowder
Assay≥97% (enzymatic)

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