4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

4-Nitrophenyl phosphate disodium salt hexahydrate, tablet

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Application


4-Nitrophenyl phosphate disodium salt hexahydrate has been used in enzyme-linked immunosorbent assay (ELISA) as a substrate for streptavidin-Alkaline Phosphatase. It has also been used as a substrate in control assay for comparing 4-aminophenyl phosphate/diethanolamine (APP/DEA) assay for alkaline phosphatase.


Biochem/physiol Actions


4-Nitrophenyl phosphate disodium salt hexahydrate (pNPP) is a non-proteinaceous and non-specific substrate for determining protein tyrosine phosphatase activity. Hydrolysis of pNPP yields p-nitrophenol. pNPP is a common chromogenic substrate for alkaline phosphatase.


General description


Chromogenic substrate for the determination of acid and alkaline phosphatases. The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.


Quantity


Contains 15 mg substrate per tablet

Quality Level200
InChI keyKAKKHKRHCKCAGH-UHFFFAOYSA-L
InChI1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitywater: 1 tablet/3 mL, clear, colorless
Formtablet

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