4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, >=97.0% (enzymatic)

4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, >=97.0% (enzymatic)

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Application


4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.


Biochem/physiol Actions


4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Quality Level100
InChI keyXXAXKCWOTRABOW-UHFFFAOYSA-N
InChI1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
ManufacturerSIGMA-ALDRICH
Impurities≤0.1% free nitrophenol, ≤6% water
Formpowder or crystals
Assay≥97.0% (enzymatic)

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