4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate

Stock Code: 3578552
Manufacturer Part No: N3129-1G
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Application


4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.


Biochem/physiol Actions


p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.


Packaging


1, 5, 25, 100 g in poly bottle

Quality Level300
InChI keyKXNNUYCXODNFBD-UHFFFAOYSA-N
InChI1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitywater: 10 mg/mL, clear, colorless to faintly yellow
Formpowder
Assay≥97% anhydrous basis

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