4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate
Application
4-Nitrophenyl 4-guanidinobenzoate hydrochloride has been used:as a substrate for trypsin for active site titration experimentsfor pre-treating of mosquito eggs in the interplasmid transposition assay as a component of isotonic buffer to moisten filter paper for mosquito embryo collection
Biochem/physiol Actions
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.
General description
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a trypsin substrate. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assay.
Packaging
1 g in glass bottle
500 mg in glass bottle
| Quality Level | 200 |
|---|---|
| InChI key | PKSBDZOBYIKNGY-UHFFFAOYSA-N |
| InChI | 1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | formic acid: soluble 49.00-51.00 mg/mL |
| Form | powder |
| Assay | ≥95.0% (TLC) |
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