4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, suitable for fluorescence, >=99.0% (TLC)

Stock Code: 3577974
Manufacturer Part No: 69585-250MG
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Application


4-Methylumbelliferyl N-acetyl-?-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to measure the total activity of ?-N-acetylhexosaminidase (NAGase) in water sample filtrates.


Biochem/physiol Actions


4-Methylumbelliferyl-N-acetyl-?-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases. It consists of chitin monomer (N-acetyl-?-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the ?-N-acetylhexosaminidase (NAGase) activity.


Other Notes


Assay of ?-N-acetylhexosaminidase

Quality Level100
InChI keyPAVCYMSNMRWMAK-DMYIEBNJSA-N
InChI1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-
ManufacturerSIGMA-ALDRICH
Impurities≤0.1% free 4-methylumbelliferone
Formpowder
Assay≥99.0% (TLC)

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