4-Chloro-1-naphthol, tablet
Application
4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen?antibody-reactive bands following western blotting.
Quantity
Contains 30 mg substrate per tablet.
Reconstitution
Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 µl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.
Specificity
4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.
| Quality Level | 200 |
|---|---|
| mp | 118-121 °C (lit.) |
| InChI key | LVSPDZAGCBEQAV-UHFFFAOYSA-N |
| InChI | 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | −20°C |
| Solubility | methanol: 10 mL, clear, colorless |
| Form | tablet |
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