3,3,5,5-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3?,5,5?-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.
| Quality Level | 200 |
|---|---|
| mp | 168-171 °C (lit.) |
| InChI key | UAIUNKRWKOVEES-UHFFFAOYSA-N |
| InChI | 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Form | tablet |
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