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3,3,5,5-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA, peroxidase substrate
3,3,5,5-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA, peroxidase substrate

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3,3,5,5-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA, peroxidase substrate

Stock Code: 3578787
Manufacturer Part No: T4319-4X100ML

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Descriptions

Application

3,3?,5,5?-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA has been used as a substrate for anti-bovine IgG-peroxidase conjugated antibody and for horse radish peroxidase-conjugatedmouseanti-mouse IgG in Poly (ADP-ribose) binding assays.

Contains TMB in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 25% slower than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Specifications

Quality Level	300
Manufacturer	SIGMA-ALDRICH
Storage Temp.	2-8°C
Form	liquid

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beta-Hydroxypyruvic acid, >=95.0% (dry substance, T)
D-(-)-Citramalic acid lithium salt, >=95.0% (GC)
Guanosine 5-diphospho-alpha-D-mannose disodium salt, >=95.0% (HPLC)
5-Bromo-4-chloro-3-indolyl alpha-D-galactopyranoside, >=98.0% (HPLC)
Kaempferol 7-O-beta -D-glucopyranoside, >=90.0% (HPLC)
L-2-Phosphoglyceric acid disodium salt hydrate, >=80% (CE)
Lipase Substrate, >=95% (HPLC)
(+/-)-Sodium 2,3-dihydroxyisovalerate hydrate, >=95% (CE)
p-Nitrophenyl-beta-D-ribofuranoside, >=98.0% (HPLC)
S-(5-Adenosyl)-3-thiopropylamine, >=98.0% (HPLC)
5-Bromo-4-chloro-3-indolyl beta-D-ribofuranoside, >=98.0% (HPLC)
4-Hydroxy-L-isoleucine, >=98.0% (TLC)
Chlorophenol Red-beta-D-galactopyranoside, >=90% (HPLC)
4-Nitrophenyl dodecanoate, >=98.0% (GC)
Lipase Substrate, for the titrimetric determination of enzyme activity
4-O-Caffeoylquinic acid, >=98.0%
4-O-Methyl-D-glucurono-D-xylan dyed with Remazol brilliant blue R, dye basis, 15% dye basis
4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, suitable for fluorescence, >=99.0% (TLC)
4-Methylumbelliferyl-beta-D-glucuronide hydrate, suitable for fluorescence, >=99.0% (HPLC)
4-Nitrophenyl phosphate disodium salt hexahydrate, for enzyme immunoassay, >=99.0% (enzymatic)
2-Phosphoglycolic acid lithium salt; greater than =95.0% (HPLC)
L-(-)-Glyceraldehyde, >=95.0% (HPLC)
4-Nitrophenyl beta-D-glucuronide, >=99.0% (TLC)
4-Nitrophenyl alpha-D-maltohexaoside, >=98.0% (HPLC)
4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, >=97.0% (enzymatic)
5-Bromo-4-chloro-3-indolyl alpha-D-glucopyranoside, >=98.0% (HPLC)
4-Methylumbelliferyl oleate, suitable for fluorescence, >=95% (HPCE)
Salvinorin B, >=93.0% (HPLC)
L-Ribulose, >=90% (HPLC)
D-Ribulose solution, approx.1 M in H2O, >=97.0% (HPLC)
Peptidoglycan from Staphylococcus aureus, cell wall component
Phenyl-beta-D-glucuronide, >=99.0% (HPLC)
(+/-)-Mevalonic acid 5-phosphate trilithium salt hydrate, 95% (TLC)
3,3,5,5-Tetramethylbenzidine, >=98.0% (NT)
3,3,5,5-Tetramethylbenzidine dihydrochloride hydrate, >=98.0% (calc. on dry substance, AT)
Tetrazolium Blue Chloride, indicator for germination, for microbiology, >=90% (T)
MTT Formazan, MTT reduction end product
4-Phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-D-Arg trifluoroacetate salt, >=95% (HPLC)
L-alpha-Hydroxyglutaric acid disodium salt, >=98.0% (GC)
Azo-Galactomannan from carob, galactomannanase substrate
2-Chloro-4-nitrophenyl-alpha-D-maltotrioside, >=96.0% (HPLC)
Neochlorogenic acid, from Lonicera japonica, >=98.0% (HPLC)
Adenosine 3-phosphate 5-phosphosulfate triethylammonium salt, >=95.0% (HPLC)
DL-alpha-Hydroxyglutaric acid disodium salt, >=98.0% (GC)
Z-Gly-Pro-4-nitroanilide, >=99.0% (TLC)
6-Chloro-3-indolyl alpha-D-galactopyranoside, >=98.0% (HPLC)
3-Dehydroquinic acid potassium salt, >=97.0% (TLC)
N-(4-Aminobutyl)-N-ethylisoluminol, >=90%
Lithium potassium acetyl phosphate, high-energy phosphate donor
2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, >=98% (HPLC)
L-Arginine-7-amido-4-methylcoumarin hydrochloride, cathepsin H substrate
Azoalbumin, protease substrate
Azocasein, protease substrate
Arg-Arg beta-naphthylamide trihydrochloride,
5-Aminosalicylic acid, >=99%
Alizarin-3-methyliminodiacetic acid,
L-Alanine 7-amido-4-methylcoumarin trifluoroacetate salt, aminopeptidase substrate
Azo dye-impregnated collagen, collagenase substrate
L-Arginine p-nitroanilide dihydrochloride, protease substrate
Luminol sodium salt,
Acetylthiocholine chloride, >=99% (TLC), powder
Acetylthiocholine iodide, >=98% (TLC), powder or crystals
3-Amino-9-ethylcarbazole, >=95%, powder
O-Acetyl-L-carnitine hydrochloride, >=99% (titration), powder
3-Amino-9-ethylcarbazole, tablet
Acetyl-Lys-D-Ala-D-Ala, >=95% (HPLC)
4-Aminophthalhydrazide,
Luminol, >=97% (HPLC)
L-Alanine 4-nitroanilide hydrochloride, protease substrate
Albumin-fluorescein isothiocyanate conjugate, albumin bovine
7-Amino-4-methylcoumarin, Chromophore for substrates
2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, tablet, 10 mg substrate per t
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, tablet
5-Bromo-4-chloro-3-indolyl sulfate potassium salt, sulfatase substrate
Resorufin benzyl ether, CYP450 substrate
N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride, protease substrate
Nalpha-Benzoyl-L-arginine 4-nitroanilide hydrochloride, >=99% (TLC), suitable for substrate for tryp
Nalpha-Benzoyl-L-arginine, >98% (TLC)
5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, >=98%, powder
5-Bromo-4-chloro-3-indolyl acetate, esterase substrate
5-Bromo-3-indolyl beta-D-galactopyranoside, >=98%
Nalpha-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate
5-Bromo-4-chloro-3-indolyl beta-D-glucopyranoside, >=97%
Boc-Leu-Ser-Thr-Arg-7-amido-4-methylcoumarin, protein C substrate
5-Bromo-4-chloro-3-indolyl alpha-D-N-acetylneuraminic acid sodium salt, >=90%
Nalpha-Benzoyl-DL-arginine beta-naphthylamide hydrochloride, trypsin substrate
Nalpha-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, >=98%
5-Bromo-4-chloro-3-indolyl beta-D-glucuronide sodium salt, >=98%
SIGMAFAST BCIP/NBT, tablet
5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, tablet
5-Bromo-4-chloro-3-indolyl phosphate disodium salt, >=98% (HPLC)
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, BioReagent, for molecular biology, powder, >=
Nalpha-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride, protease substrate
N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate
6-Bromo-2-naphthyl beta-D-glucopyranoside, >=99% (TLC)
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, >=99% (HPLC)
5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside, >=98%
5-Bromo-4-chloro-3-indolyl beta-D-fucopyranoside, >=98% (HPLC)
Casein fluorescein isothiocyanate from bovine milk, Type III, essentially salt-free, lyophilized pow
Cellulose Azure, cellulase substrate
Chitin azure, chitinase substrate
Casein fluorescein isothiocyanate from bovine milk, Type II, essentially salt-free, lyophilized powd
beta-Chloro-D-alanine hydrochloride,
4-Chloro-1-naphthol, tablet
4-Chloro-1-naphthol, crystalline
beta-Chloro-L-alanine hydrochloride, Alanine aminotransferase inhibitor
Z-Phe-Arg 7-amido-4-methylcoumarin hydrochloride, kallikrein substrate
Chemiluminescent Peroxidase Substrate-3
SIGMAFAST DAB with Metal Enhancer, tablet
3-(Dimethylamino)benzoic acid, >=97% (HPLC)
1-Fluoro-2,4-dinitrobenzene, >=99%
SIGMAFAST 3,3-Diaminobenzidine tablets, tablet, To prepare 5 mL
SIGMAFAST 3,3-Diaminobenzidine tablets, tablet, To prepare 15 mL
3,3-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet
o-Dianisidine, peroxidase substrate
o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 10 mL
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL
5-Fluoroorotic acid hydrate, >=98%
Farnesyl pyrophosphate ammonium salt, methanol:ammonia solution, >=95% (TLC)
Fast Green FCF, Dye content >=85 %
Fluorescamine, >=98% (TLC), powder, used for detection of primary amines
L-Glutamic acid gamma-(4-methoxy-beta-naphthylamide),
Gly-Phe p-nitroanilide, cathepsin c substrate
Gly-Pro p-nitroanilide hydrochloride, >=99% (TLC)
L-Glutamic acid gamma-(4-nitroanilide), gamma-glutamyl transpeptidase substrate
N-Glutaryl-L-phenylalanine p-nitroanilide, protease substrate
Gly-Pro p-nitroanilide p-toluenesulfonate salt, approx.99% (TLC)
L-Glutamic acid gamma-(beta-naphthylamide),
Glutaryl-Ala-Ala-Phe-4-methoxy-beta-naphthylamide, protease substrate
Guanosine 5-diphospho-beta-L-fucose sodium salt, >=85%
L-alpha-Glycerophosphorylcholine, from soybean, >=98%, powder
L-Glutamic acid gamma-(p-nitroanilide) hydrochloride, gamma-glutamyl transpeptidase substrate
Geranyl pyrophosphate ammonium salt, 1 mg/mL in methanol (:aqueous 10 mM NH4OH (7:3)), >=95% (TLC)
Gly-Pro 4-methoxy-beta-naphthylamide, dipeptidylpeptidase?ßIV substrate
3-Hydroxy-DL-kynurenine,
Hippuryl-Arg, carboxypeptidase substrate
Hide-Remazol Brilliant Blue R, protease substrate
DL-beta-Hydroxybutyric acid sodium salt, approx.98%
Hippuryl-L-phenylalanine,
4-Hydroxybenzhydrazide, >=97%
Indoxyl phosphate disodium salt, crystalline
Idebenone, >=98% (HPLC)
Iodonitrotetrazolium violet-formazan, crystalline
alpha-Ketoglutaric acid, BioReagent, suitable for cell culture, suitable for insect cell culture
alpha-Ketoglutaric acid, >=98.5% (NaOH, titration)
alpha-Ketoglutaric acid sodium salt, >=98% (enzymatic)
alpha-Ketoglutaric acid potassium salt, >=98% (enzymatic)
alpha-Ketoglutaric acid sodium salt, BioUltra
Kynuramine dihydrobromide, crystalline
L-Leucine beta-naphthylamide hydrochloride, leucine aminopeptidase substrate
L-Leucine beta-naphthylamide,
L-Leucine-7-amido-4-methylcoumarin hydrochloride,
L-Leucine p-nitroanilide hydrochloride, >=98% (TLC)
D-Luciferin, synthetic, BioXtra, >=99% (HPLC)
D-Luciferin sodium salt, lyophilized powder, synthetic
L-Leucine-p-nitroanilide, leucine aminopeptidase substrate
D-Luciferin, synthetic
5-Methyltetrahydrofolic acid disodium salt, >=88% (UV-vis)
Methylglyoxal solution, approx. 40% in H2O
4-Methylumbelliferyl beta-D-mannopyranoside, >=98% (TLC)
4-Methylumbelliferone sodium salt, >=98% (HPLC), crystalline
4-Methylumbelliferyl beta-D-galactopyranoside, >=99% (TLC)
4-Methylumbelliferyl N-acetyl-beta-D-glucosaminide, beta-N-acetylhexosaminidase substrate
4-Methylumbelliferyl beta-D-lactopyranoside, beta-galactosidase substrate
4-Methylumbelliferyl alpha-L-arabinopyranoside, >=99% (HPLC)
4-Methylumbelliferyl beta-D-glucopyranoside, beta-glucosidase substraTE
4-Methylumbelliferyl alpha-D-mannopyranoside, >=97% (HPLC)
4-Methylumbelliferyl p-trimethylammoniocinnamate chloride, powder
N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide, elastase substrate
1-Methylhistamine dihydrochloride, >=98% (TLC), powder
4-Methylumbelliferyl beta-D-N,N,N-triacetylchitotrioside, fluorogenic glycanase substrate
4-Methylumbelliferyl beta-D-cellobioside, glucanase substrate
4-Methylumbelliferyl-beta-D-xylopyranoside, beta-xylosidase substrate
4-Methylumbelliferyl sulfate potassium salt, sulfatase substrate
4-Methylumbelliferyl alpha-D-galactopyranoside, >=98% (TLC)
4-Methylumbelliferyl phosphate disodium salt, phosphatase substrate
4-Methylumbelliferyl alpha-L-fucopyranoside, >=97% (TLC)
2-(4-Methylumbelliferyl)-alpha-D-N-acetylneuraminic acid sodium salt hydrate, >=95% (HPLC)
4-Methylumbelliferyl phosphate, phosphatase substrate
4-Methylumbelliferyl N-acetyl-beta-D-galactosaminide, >=98% (HPLC)
4-Methylumbelliferyl beta-D-N,N-diacetylchitobioside hydrate, >=98% (TLC)
4-Methylumbelliferyl alpha-D-glucopyranoside, alpha-glucosidase substrate
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, elastase substrate
4-Nitrophenyl decanoate, lipase substrate
Naphthol AS-D chloroacetate, esterase substrate
4-Nitrophenyl alpha-D-galactopyranoside, alpha-galactosidase substrate
4-Nitrophenyl beta-D-galacto?ípyran?íoside, (enzymatic), >=98% (enzymatic)
4-Nitrophenyl beta-D-mannopyranoside, >=98%
4-Nitrophenyl alpha-D-glucopyranoside, >=99%
2-O-(p-Nitrophenyl)-alpha-D-N-acetylneuraminic acid, >=95%
4-Nitrophenyl beta-D-lactopyranoside, powder
Naphthol AS-BI beta-D-glucuronide, beta-glucuronidase substrate
SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
4-Nitrophenyl alpha-D-xylopyranoside, alpha-xylosidase substrate
4-Nitrophenyl alpha-D-mannopyranoside, alpha-mannosidase substrate
4-Nitrophenyl beta-D-xylopyranoside, >=98%
Naphthol AS-BI phosphate disodium salt hydrate, >=99.0% (HPLC)
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
4-Nitrophenyl palmitate, lipase substrate
SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL
Bis(p-nitrophenyl) phosphate sodium salt, >=99% (TLC)
p-Nitrophenyl galacto-N-bioside, >=98%
4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
4-Nitrophenyl phosphate di(tris) salt, phosphatase substrate
4-Nitrophenyl beta-D-fuco?ípyran?íoside, >=98% (TLC)
4-Nitrophenyl alpha-L-fuco?ípyran?íoside, >=98% (TLC)
4-Nitrophenyl alpha-L-arabinofuranoside, >=98% (TLC)
Potassium 4-nitrophenyl sulfate, sulfatase substrate
Naphthol AS-BI N-acetyl-beta-D-glucosaminide, beta-hexosaminidase substrate
4-Nitrophenyl N-acetyl-alpha-D-galactosaminide, N-acetyl-alpha-D-galactosaminidase substrate
4-Nitrophenyl valerate, liquid
2-Nitrophenyl beta-D-cellobioside, butyrylcholinesterase inhibitor
Naphthol AS-MX phosphate, powder, >=99% (HPLC)
4-Nitrophenyl beta-D-cellobioside, >=98% (TLC)
Naphthol AS-TR phosphate disodium salt, >=99% (HPLC), Bulk package
4-Nitrophenyl N,N-diacetyl-beta-D-chitobioside, >=99% (TLC)
4-Nitrophenyl beta-D-glucopyranoside, >=98% (TLC)
1-Naphthyl phosphate disodium salt, phosphatase substrate
2-Naphthyl phosphate sodium salt,
4-Nitrophenyl alpha-L-rhamnopyranoside, powder
1-Naphthyl butyrate, esterase substrate
4-Nitrophenyl 4-guanidinobenzoate hydrochloride, protease inhibitor and substrate
2-Nitrophenyl beta-D-glucopyranoside, powder
4-Nitrophenyl acetate, esterase substrate
P-nitrophenyl acetate
4-Nitrophenyl N-acetyl-alpha-D-glucosaminide, >=98%
6-Nitro-3-(phenylacetamido)benzoic acid, >=98%
4-Nitrophenyl N-acetyl-beta-D-galactosaminide, >=98%
4-Nitrophenyl N-acetyl-beta-D-glucosaminide, >=99% (TLC)
4-Nitrophenyl butyrate, >=98%
Oxaloacetic acid, >=97%
Phenolphthalein beta-D-glucuronide sodium salt, beta-glucuronidase substrate
Phosphocholine chloride calcium salt tetrahydrate, Sigma Grade
Phenolphthalein beta-D-glucuronide, beta-glucuronidase substrate
o-Phenylenediamine dihydrochloride, tablet, 60 mg substrate per tablet
pGlu-Phe-Leu p-nitroanilide, protease substrate
o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet
Phe-Arg beta-naphthylamide dihydrochloride, cathepsin substrate
o-Phenylenediamine dihydrochloride, tablet, 15 mg substrate per tablet
Phosphatase substrate
Phosphatase substrate, powder
o-Phenylenediamine, tablet, 20 mg substrate per tablet
Phenolphthalein monophosphate bis(cyclohexylammonium) salt, powder, >=85% (TLC)
Phosphatase substrate, 100 mg capsules
o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet
o-Phenylenediamine dihydrochloride, tablet, 2 mg substrate per tablet
Phospho(enol)pyruvic acid trisodium salt hydrate, >=97% (enzymatic)
Phospho(enol)pyruvic acid monopotassium salt, >=97% (enzymatic)
Phospho(enol)pyruvic acid tri(cyclohexylammonium) salt, >=98% (enzymatic)
o-Phenylenediamine dihydrochloride, tablet, 20 mg substrate per tablet
Phosphocreatine disodium salt hydrate, >=97%
o-Phenylenediamine dihydrochloride, tablet, 10 mg substrate per tablet
o-Phenylenediamine dihydrochloride, tablet, 30 mg substrate per tablet
o-Phenylenediamine dihydrochloride, tablet, 4 mg substrate per tablet
o-Phenylenediamine dihydrochloride, tablet, 3 mg substrate per tablet
SIGMAFAST OPD, tablet
RNase B Glycoprotein Standard from bovine pancreas, Proteomics Grade
Resorufin beta-D-glucuronide sodium salt, >=90%
Resorufin beta-D-galactopyranoside, approx.95%
N-Succinyl-Ala-Phe-Lys 7-amido-4-methylcoumarin acetate salt,
Phosphatase substrate, 5 mg tablets
N-Succinyl-Leu-Tyr-7-amido-4-methylcoumarin, calpain substrate
N-Succinyl-Gly-Gly-Phe-p-nitroanilide, protease substrate
N-Succinyl-L-phenylalanine-p-nitroanilide, protease substrate
N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate
Ser-Tyr beta-naphthylamide, powder
Starch Azure, Potato starch covalently linked with Remazol Brilliant Blue R
Syringaldazine, indicator for laccase and peroxidase activity
N-Succinyl-Ala-Ala-Pro-Leu p-nitroanilide, protease substrate
N-Succinyl-Ala-Ala-Phe-7-amido-4-methylcoumarin, protease substrate
Sar-Pro-Arg p-nitroanilide dihydrochloride, thrombin substrate
N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, Leucine aminopeptidase substrate
3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrate
L-Tyrosine 7-amido-4-methylcoumarin,
3,3,5,5-Tetramethylbenzidine, >=98% (TLC)
3,3,5,5-Tetramethylbenzidine Liquid Substrate, Slow Kinetic Form, for ELISA, peroxidase substrate
3, 3,5 ,5-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA, ready to use solution
Thymidine 5-monophosphate p-nitrophenyl ester sodium salt, >=98% (HPLC)
3,3,5,5-Tetramethylbenzidine, tablet, 1 mg substrate per tablet
N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt, plasmin substrate
3,3,5,5-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate
3,3,5,5-Tetramethylbenzidine dihydrochloride hydrate, powder, >=98%
TMB Enhanced One Component HRP Membrane Substrate,
D-Val-Leu-Lys 4-nitroanilide dihydrochloride, plasmin substrate
D-Val-Leu-Lys p-nitroanilide dihydrochloride, plasmin substrate
Hematoxylin,