3,3,5,5-Tetramethylbenzidine dihydrochloride hydrate, powder, >=98%
Application
Noncarcinogenic substitute for benzidine.
3,3?,5,5?-Tetramethylbenzidine dihydrochloride hydrate has also been used as a horseradish peroxidase substrate in enzyme linked immunosorbent assay and reverse dot blot (RDB).
General description
3,3?,5,5?-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes.
| Quality Level | 200 |
|---|---|
| InChI key | KVCWTKDFVVSVSJ-UHFFFAOYSA-N |
| InChI | 1S/C16H20N2.2ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;;/h5-8H,17-18H2,1-4H3;2*1H;1H2 |
| Manufacturer | SIGMA-ALDRICH |
| Storage Temp. | 2-8°C |
| Solubility | acetic acid: water (1:2): 20 mg/mL, clear to slightly hazy |
| Form | powder |
| Assay | ≥98% |
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