3,3-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

3,3-Diaminobenzidine tetrahydrochloride, tablet, 10 mg substrate per tablet

Stock Code: 3578265
Manufacturer Part No: D5905-5X100TAB
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Application


Reagent for spectrophotometric determination of selenium.


3,3?-Diaminobenzidine tetrahydrochloride has been used for immunohistochemistry.


Biochem/physiol Actions


3,3?-Diaminobenzidine tetrahydrochloride is a chromogenic substrate, which is commonly used in immunoperoxidase tests.


Reconstitution


Dissolve 1 tablet in 15 ml of Tris-buffered saline, pH 7.6. Add 12 µl of fresh 30% hydrogen peroxide. DAB and hydrogen peroxide concentration may be adjusted to suit individual applications.


Specificity


Peroxidase substrate suitable for use in immunoblotting and immunohistological staining procedures. This substrate produces an insoluble end product that is brown in color and can be observed visually.

Quality Level200
InChI keyKJDSORYAHBAGPP-UHFFFAOYSA-N
InChI1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitywater: 1 tablet/15 mL (may contain small insoluble particles)
Formtablet

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