1-Fluoro-2,4-dinitrobenzene, >=99%

Stock Code: 3578254
Manufacturer Part No: D1529-25ML

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Descriptions

Application

1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.

Biochem/physiol Actions

1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.

General description

1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†

Packaging

10, 25, 100 mL in glass bottle

Specifications

Quality Level	200
mp	25-27 °C (lit.)
InChI key	LOTKRQAVGJMPNV-UHFFFAOYSA-N
InChI	1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
bp	178 °C/25 mmHg (lit.)
Manufacturer	SIGMA-ALDRICH
Density	1.482 g/mL at 25 °C (lit.)
Form	solid
Assay	≥99%