Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut

Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco’;s Modified Eagle’;s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove’;s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Reconstitution

Supplement with 0.584 g/L L-glutamine

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Code Description Quality Level Pack Manufacturer Technique(s) Storage Temp. Impurities Form Price Quantity
3577088 Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut 400 1L SIGMA-ALDRICH cell culture - hybridoma: suitable, cell culture - mammalian: suitable 2-8°C endotoxin, tested liquid
£81.72 (exc VAT) per 1L
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3577089 Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut 400 500ML SIGMA-ALDRICH cell culture - hybridoma: suitable, cell culture - mammalian: suitable 2-8°C endotoxin, tested liquid
£38.91 (exc VAT) per 500ML
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