Minimum Essential Medium Eagle, With Earles salts, L-glutamine and sodium bicarbonate, liquid, steri

Descriptions

Application

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle’;s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks’; or Earle’;s salts has broadened the usefulness of this medium.

Other Notes

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Pack	Manufacturer	Storage Temp.	Impurities	Form	Price		Quantity
3577107	Minimum Essential Medium Eagle, With Earles salts, L-glutamine and sodium bicarbonate, liquid, steri	300	1L	SIGMA-ALDRICH	2-8°C	endotoxin, tested	liquid	£59.30 (exc VAT) per 1L		- +
3577108	Minimum Essential Medium Eagle, With Earles salts, L-glutamine and sodium bicarbonate, liquid, steri	300	500ML	SIGMA-ALDRICH	2-8°C	endotoxin, tested	liquid	£30.70 (exc VAT) per 500ML		- +