Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut

Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut

Stock Code: 3577088
Manufacturer Part No: I3390-1L
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Application


Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco’;s Modified Eagle’;s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove’;s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.


Reconstitution


Supplement with 0.584 g/L L-glutamine

Quality Level400
Pack1L
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - hybridoma: suitable, cell culture - mammalian: suitable
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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