Iscoves Modified Dulbeccos Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glut
Application
Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco’;s Modified Eagle’;s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove’;s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Reconstitution
Supplement with 0.584 g/L L-glutamine
| Quality Level | 400 |
|---|---|
| Pack | 500ML |
| Manufacturer | SIGMA-ALDRICH |
| Technique(s) | cell culture - hybridoma: suitable, cell culture - mammalian: suitable |
| Storage Temp. | 2-8°C |
| Impurities | endotoxin, tested |
| Form | liquid |
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