Medium 199, 10 x, With Hanks salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filt
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Reconstitution
Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.
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