Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and s

Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and s

Application

Minimum Essential Medium Eagle is used for primary osteoblast cultures and for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Minimum Essential Medium Eagle has been used for the isolation of mesenchymal stem cell, adipose-derived stem cell, and osteoblast.

Biochem/physiol Actions

MEM usually autoclaved with 0.5% bactopeptone at 15 psi for 20 min, cooled and then neutralized with NaHCO3, consistently supported good cell growth. A similar result was obtained when the MEM and bactopeptone were autoclaved separately. The cells grow, initially, as a monolayer and subsequently become a stationary suspension.

General description

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.This is the most enriched variation of the MEM formulation offered. It contains all 21 normal amino acids, some at increased concentrations. In addition, it contains 5 additional vitamins. Often used in cytogenetic analysis when supplemented with fetal bovine serum (FBS).

Reconstitution

Supplement with 0.292 g/L L-glutamine

No detailed specifications are available for this product.

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Code Description Quality Level Pack Manufacturer Technique(s) Storage Temp. Impurities Form Price Quantity
3577121 Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and s 400 - SIGMA-ALDRICH cell culture - mammalian: suitable 2-8°C endotoxin, tested liquid
£960.11 (exc VAT) per EACH
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3577122 Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and s 400 500ML SIGMA-ALDRICH cell culture - mammalian: suitable 2-8°C endotoxin, tested liquid
£64.01 (exc VAT) per 500ML
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+
3577123 Minimum Essential Medium Eagle, Alpha Modification, with ribonucleosides, deoxyribonucleosides and s 400 6X500ML SIGMA-ALDRICH cell culture - mammalian: suitable 2-8°C endotoxin, tested liquid
£306.57 (exc VAT) per EACH
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