Medium 199, With Hanks salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered,

Stock Code: 3577118
Manufacturer Part No: M7653-6X500ML
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General description


Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.


Modification of the original Medium 199.Earle′s salts are replaced with Hanks′ salts. Hanks′ salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.


Reconstitution


Supplement with 0.1 g/L L-glutamine.

Quality Level400
Pack6X500ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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