Dulbeccos Modified Eagles Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, with

Stock Code: 3577035
Manufacturer Part No: D5671-500ML

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Descriptions

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

General description

This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine, HEPES, or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.

Reconstitution

Supplement with 0.584 g/L L-glutamine.

Specifications

Quality Level	300, 400
Pack	500ML
Manufacturer	SIGMA-ALDRICH
Technique(s)	cell culture - hybridoma: suitable, cell culture - mammalian: suitable
Storage Temp.	2-8°C
Impurities	endotoxin, tested
Form	liquid