SSC Buffer, powder blend for hybridization buffers
Application
Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots
Components
SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.
Convenient pack sizes Multi-purpose use in hybridizations Easy-to-use powder makes fast, inexpensive solutions
General description
SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.
Preparation Note
Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.
| Quality Level | 100 |
|---|---|
| Manufacturer | SIGMA-ALDRICH |
| Product Line | BioReagent |
| Storage Temp. | room temp |
| Impurities | phosphatases, none detected, RNases, none detected, DNases, none detected, proteases, none detected |
| Solubility | H2O: 0.263 g/mL, clear, colorless |
| Form | powder blend |
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