The Transcreener® UDP2 FP Assay is a universal biochemical HTS assay for enzymes that produce UDP, including glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetlygalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose and hyaluronan synthases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® UDP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 1000 μM. The assay provides excellent signal at low substrate conversion, with a Z’ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.View full Transcreener® product list
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