SSC Buffer, powder blend for hybridization buffers

SSC Buffer, powder blend for hybridization buffers

Application

Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots

Components

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Convenient pack sizes Multi-purpose use in hybridizations Easy-to-use powder makes fast, inexpensive solutions

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

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Code Description Quality Level Manufacturer Product Line Storage Temp. Impurities Solubility Form Price
3581779 SSC Buffer, powder blend for hybridization buffers 100 SIGMA-ALDRICH BioReagent room temp phosphatases, none detected, RNases, none detected, DNases, none detected, proteases, none detected H2O: 0.263 g/mL, clear, colorless powder blend
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3581780 SSC Buffer, powder blend for hybridization buffers 100 SIGMA-ALDRICH BioReagent room temp phosphatases, none detected, RNases, none detected, DNases, none detected, proteases, none detected H2O: 0.263 g/mL, clear, colorless powder blend
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