Guanidine thiocyanate, BioReagent, for molecular biology

Descriptions

Application

Chaotropic agent and strong denaturant; solubilizes cells.

Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) plays a role in protein denaturation. It is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures ribonuclease (RNase). GTC has been studied in the unfolding and refolding of fluorescent proteins (FP). It can be used along with phenol and chloroform for extraction of RNA.

Packaging

100, 500 g in glass bottle

Principle

Guanidine thiocyanate interferes with the hydrogen bonds and intramolecular interactions of macromolecules. It solubilizes the proteins resulting in disintegration of cellular structures.

Specifications

No detailed specifications are available for this product.

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Code	Description	Colour	Manufacturer	Impurities	Form	Assay	Price		Quantity
3581765	Guanidine thiocyanate, BioReagent, for molecular biology	colorless to white,	SIGMA-ALDRICH	phosphatases, none detected, RNases, none detected, proteases, none detected, DNases, none detected	powder	≥99%	£161.25 (exc VAT) per 100G		- +
3581766	Guanidine thiocyanate, BioReagent, for molecular biology	colorless to white,	SIGMA-ALDRICH	phosphatases, none detected, RNases, none detected, proteases, none detected, DNases, none detected	powder	≥99%	£557.50 (exc VAT) per 500G		- +