TRANSCREENER(TM) EPIGEN METHYLTRANSFERAS

TRANSCREENER(TM) EPIGEN METHYLTRANSFERAS

General description

The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener® AMP2/GMP2 Assay, which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener® AMP2/GMP2 Antibody. The assay is a simple mix-and-read format with two liquid addition steps. Methyltransferase (MT) enzyme reactions are first quenched with Stop Buffer and then the SAH Detection Mixture containing coupling enzymes, Transcreener® AMP2/GMP2 antibody, and tracer is added. The assay provides excellent signal at low substrate conversion, with a Z’; =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.View full Transcreener® product list

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Physical form

Kit with buffered aqueous solutions

Quantity

3017-1K = 1,000 assay, 384-well3017-10K = 10,000 assay, 384-well

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Code Description Manufacturer Storage Temp. Price Quantity
3581747 TRANSCREENER(TM) EPIGEN METHYLTRANSFERAS SIGMA-ALDRICH −20°C
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3581748 TRANSCREENER(TM) EPIGEN METHYLTRANSFERAS SIGMA-ALDRICH −20°C
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