Pyronin Y, RNA staining tested
Application
Pyronin Y has been used: in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)staining of CD34+ cells
Biochem/physiol Actions
Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.
Packaging
1, 5 g in glass bottle
Preparation Note
This product is soluble in water 1 mg/ml, yielding a clear dark red solution.
| Quality Level | 200 |
|---|---|
| mp | 250-260 °C (lit.) |
| InChI key | INCIMLINXXICKS-UHFFFAOYSA-M |
| InChI | 1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1 |
| Manufacturer | SIGMA-ALDRICH |
| Composition | Dye content, ≥45% |
| Form | powder |
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