Nitrotetrazolium Blue chloride, powder, for molecular biology
Application
Nitrotetrazolium Blue chloride has been used: to determine the superoxide anion in skeletal muscle cells as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sectionsas a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlingsas a component in Triton X-100 solution to perform staining of coronal brain sections
Biochem/physiol Actions
Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2.
General description
Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
Packaging
1 g in glass bottle
50, 250 mg in glass insert
| Quality Level | 100 |
|---|---|
| InChI key | FSVCQIDHPKZJSO-UHFFFAOYSA-L |
| InChI | 1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8- |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | ethanol: 5 mg/mL, 2-methoxyethanol: 20 mg/mL, H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.) |
| Form | powder |
| Assay | >90% |
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