Hybridization Solution, for Northern and Southern blots

Hybridization Solution, for Northern and Southern blots

Stock Code: 3581951
Manufacturer Part No: H7140-5X50ML
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Application


Suitable for use as a hybridization buffer in Northern and Southern blot protocols.


Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.


Components


Sigma’;s Hybridization Solution contains 200 µg/mL DNA in 10x SSC and 2X Denhardt’;s solutions:200 µg/mL DNA2mg Bovine Serum Albumin (BSA)2mg Ficoll2mg Polyvinylpyrrolidone (PVP)0.15 M sodium citrate1.5 M NaCl


General description


Sigma’;s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Quality Level100
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formliquid (2x concentrate)

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