Glyoxal solution, BioReagent, for molecular biology, approx. 40% in H2O (approx. 8.8 M)
Application
Suitable for nucleic acid denaturation binding of DNA/RNA to nitrocellulose membrane
Caution
May precipitate on storage; redissolve at 50-60 °C.
General description
Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.
Other Notes
This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.
May precipitate on storage; redissolve at 50-60?°C.
Principle
Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.
| InChI key | LEQAOMBKQFMDFZ-UHFFFAOYSA-N |
|---|---|
| InChI | 1S/C2H2O2/c3-1-2-4/h1-2H |
| Manufacturer | SIGMA-ALDRICH |
| Impurities | phosphatases, none detected, RNases, none detected, proteases, none detected, DNases, none detected |
| Form | liquid |
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