ROCHE NADGrade I free acid

ROCHE NADGrade I free acid

Stock Code: 2795399
Manufacturer Part No: 10127973001
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Application


NAD has been used in in vitro glycogen synthase kinase 3 (GSK3) deacetylation assay. It has also been used as a component in assay mixfor the determination of kinetic characteristics using an enzyme-linked kinetic assay.


NAD (nicotinamide adenine dinucleotide), Grade I has been used in the kinetic studies of human class IV alcohol dehydrogenase.


Biochem/physiol Actions


The NAD (nicotinamide adenine dinucleotide)/NADH ratio regulates the intracellular redox potential, thereby influencing metabolic reactions in vivo. It works as an electron acceptor.


NAD is also involved in microbial catabolism. ?-NAD acts as a substrate for various enzymes in several cellular processes.


Electron acceptor


General description


Nicotinamide adenine dinucleotide (NAD) is a ubiquitously found electron carrier and a cofactor. NAD+ contains an adenylic acid and a nicotinamide-5?-ribonucleotide group linked together by a pyrophosphate moiety. In NAD+ complexes, the enzyme-cofactor interactions are highly conserved.


Other Notes


For life science research only. Not for use in diagnostic procedures.


This is the common form of NAD.


Packaging


Packaged by solid weight.


Preparation Note


Storage conditions (working solution): 10 mg/ml in aqueous solution, contains at least 99% NAD after 4 weeks at 2 to 8 °C


Quality


Purity: 100% β-NAD based on dry weight (enzymatically, CN-complex and absorbance); A250nm - A260nm : 0.83 ± 0.02, A280nm - A260nm : 0.22 ± 0.02Absence of contaminants: LDH inhibitors are not detectableOrganic solvents: =0.05% (GC)


Specifications


Absorbance coefficients: 17.6 x 103l x mol-1 x cm-1 at 260nm, 5.9 x 103l x mol-1 x cm-1 at 333nm (CN complex)

Colouroff-white
InChI keyBAWFJGJZGIEFAR-NNYOXOHSSA-N
InChI1S/C21H27N7O14P2/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(32)14(30)11(41-21)6-39-44(36,37)42-43(34,35)38-5-10-13(29)15(31)20(40-10)27-3-1-2-9(4-27)18(23)33/h1-
Formlyophilized
Assay100% (dry weight)

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