SeqPlex RNA Amplification Kit, For use with high throughput sequencing technologies

SeqPlex RNA Amplification Kit, For use with high throughput sequencing technologies

Application

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Features and Benefits

Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP. Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming. No need to fragment DNA before sequencing. Amplifies ds-cDNA in 8 hours or less. Compatible with all next generation sequencing platforms except Pacific Bioscience.

General description

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

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Code Description Quality Level Manufacturer Technique(s) Storage Temp. Price Quantity
3582462 SeqPlex RNA Amplification Kit, For use with high throughput sequencing technologies 200 SIGMA-ALDRICH whole genome amplification: suitable −20°C
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3582463 SeqPlex RNA Amplification Kit, For use with high throughput sequencing technologies 200 SIGMA-ALDRICH whole genome amplification: suitable −20°C
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3582464 SeqPlex RNA Amplification Kit, For use with high throughput sequencing technologies 200 SIGMA-ALDRICH whole genome amplification: suitable −20°C
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