ROCHE Faststart Taq DNA Pol. DNTPack

ROCHE Faststart Taq DNA Pol. DNTPack

Application

FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR setup and prior to the initial denaturation step. It can be applied for PCR:Hot start PCR up to 3 kbHot Start RT-PCR up to 3 kbMultiplex PCRDifficult templates, e.g., secondary structures or GC-rich sequencesAutomated PCR, e.g., handling at room temperaturesquantitaive PCR(qPCR)For maximum convenience, select the 2x concentrated ready-to-use FastStart PCR Master.

Features and Benefits

Volume activity: 5 U/µlEach dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix. Higher specificity, sensitivity, and yield:Hot start PCR makes PCR setup easier. Use robotic setup.Use this enyzme mix stable for 24 hours at +15 to +25°C. Prevent PCR carryover contamination.Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes. Cost-effective.Use the convenient premixed solution of PCR grade nucleotides.

General description

The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable, recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.

Legal Information

FastStart is a trademark of Roche

NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc

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Code Description Quality Level Parameter Storage Temp. Price Quantity
2795286 ROCHE Faststart Taq DNA Pol. DNTPack 500 U 100 72 °C optimum reaction temp. −20°C
£249.32 (exc VAT) per EACH
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2795287 ROCHE Faststart Taq DNA Polymerase DNTPack 1000 U 100 72 °C optimum reaction temp. −20°C
£480.54 (exc VAT) per EACH
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2795288 ROCHE Faststart Taq DNA Pol. DNTPack 2500 U 100 72 °C optimum reaction temp. −20°C
£1,167.21 (exc VAT) per EACH
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2795289 ROCHE Faststart Taq DNA Pol. DNTPack 5000 U 100 72 °C optimum reaction temp. −20°C
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