JumpStart REDAccuTaq LA DNA Polymerase, Long and accurate hot-start Taq with inert dye, 10X buffer i

Descriptions

Application

JumpStart REDAccuTaq LA DNA Polymerase is a unique enzyme blend that is capable of generating long PCR fragments, from 0.25 kb to 40 kb, with high fidelity, increased specificity and yield. JumpStart REDAccuTaq DNA polymerase combines Sigma′s AccuTaq LA DNA polymerase and JumpStart Taq antibody with an inert red dye. This specially formulated hot start enzyme mix achieves greater yields, enhances sensitivity and results in higher fidelity (6.5×) in comparison to standard Taq or other Long and Accurate enzyme blends. Its high fidelity makes it the enzyme of choice when performing amplifications where a low error frequency is critical, such as in RT-PCR and cloning. JumpStart REDAccuTaq LA DNA Polymerase also contains the hot start mechanism of the JumpStart Taq antibody. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other hot-start methods (i.e. chemical inactivation), JumpStart Taq antibody does not require a pre-incubation step prior to cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction.The inert red dye provides quick recognition and confirmation of appropriate mixing. An aliquot of the samples (5-10 µL) may be loaded directly onto an agarose gel following PCR. The red dye migrates slightly faster than bromophenol blue at the same rate as a 125 base pair fragment.The PCR product can be easily separated from the dye by standard purification methods. The inert red dye has does not effect automated sequencing, restriction enzyme digestion, ligation or other downstream applications.

JumpStart^™REDAccuTaq^®LA DNA Polymerase has been used for amplifying genomic DNA. It has also been used in polymerase chain reaction (PCR) for gene cloning.

Features and Benefits

JumpStart REDAccuTaq LA DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity Up to 6.5X greater fidelity in comparison to Taq DNA polymerase making it the ideal enzyme for multiplex PCR Produce amplicons up to 22 kb with genomic templates and up to 40 kb with less complex templates such as lambda or bacterial genomic DNA Reduce set-up time and eliminate concerns associated with manual or wax Hot Start methods Dye allows for quick visual confirmation that reagent has been added and mixed properly Direct loading onto an agarose gel without additional dyes

General description

JumpStart^™ REDAccuTaq^® LA DNA Polymerase contains AccuTaq long and accurate (LA) DNA polymerase, an inert red dye, and JumpStart Taq antibody. This enzyme is suitable for long-distance and high-fidelity PCR, multiplex PCR, and PCR amplification of targets with variable lengths, such as amplification of cDNA libraries. It enables the amplification from 0.25 to 22 kb for complex genomic DNA and up to 40 kb for less complex templates. JumpStart Taq antibody is designed to minimize non-specific amplification while increasing target yield. Unlike other Hot-start methods (i.e. chemical inactivation), the JumpStart Taq antibody does not require a pre-incubation step before cycling because polymerase activity is fully restored during the first denaturation cycle of the PCR reaction

Specifications

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Code	Description	Colour	Quality Level	Manufacturer	Feature	Form	Price		Quantity
3582213	JumpStart REDAccuTaq LA DNA Polymerase, Long and accurate hot-start Taq with inert dye, 10X buffer i	red	200	SIGMA-ALDRICH	hotstart, dNTPs included: no, Long & Accurate PCR	liquid	£358.29 (exc VAT) per EACH		- +
3582214	JumpStart REDAccuTaq LA DNA Polymerase, Long and accurate hot-start Taq with inert dye, 10X buffer i	red	200	SIGMA-ALDRICH	hotstart, dNTPs included: no, Long & Accurate PCR	liquid	£79.20 (exc VAT) per EACH		- +