ROCHE N-Glycosidase FPNGase F of Flavobacterium meningosepticum recombinant from E. coli

Stock Code: 2795584
Manufacturer Part No: 11365169001

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Descriptions

Application

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

General description

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

Legal Information

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

Unit Definition

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.