ROCHE Liberase TH Research Grade 100 Mg

Descriptions

Application

Liberase^™ TH (Thermolysin High) Research Grade is used for the dissociation of a broad range of tissue types, where high purity of the enzyme blend is necessary for high cell yield and viability. Bacterial by-products, such as endotoxins, are reduced up to several thousand-fold.

Features and Benefits

Maximize viability and yield of isolated cellswith an enzyme blend that has less clostripain and trypsin activity, as well as reduced endotoxin content. Count on higher specific activity of the enzyme blendas a result of higher Collagenase I + II purity (determined by HPLC analysis). Obtain higher experimental reproducibilitydue to higher lot-to-lot consistency. Increase safetywith an enzyme that is free of mammalian or avian tissue-derived raw materials.

General description

Liberase TH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Thermolysin, a non-clostridial neutral protease.

Legal Information

Liberase is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Stabilizers: CalciumWorking concentration: Liberase Research Grade Enzyme Working Concentration:Liberase enzymes have significantly higher specific activities than traditional collagenases. This means that the working concentration of Liberase Research Grade Purified Enzymes, expressed in mg/ml, will be lower than that of traditional collagenase.When the application is on the Roche list of applications at www.collagenase.com, use the Liberase Research Grade concentration recommended for that application.When the application is not included on this list, first use Liberase TM Research Grade at a concentration of 0.08–0.28 WÃ¼nsch units/ml.The goal is to determine the best starting concentration of Liberase Research Grade Enzyme Blends. This is a starting point, and the final concentration may vary due to differences in procedure and lot-to-lot differences in traditional collagenase.Collagenase Working Concentration:Multiply your previous collagenase working concentration (mg/ml) by its specific activity (WÃ¼nsch units/mg, [as determined above]), to obtain WÃ¼nsch units/ml. To determine how much Liberase Research Grade Enzyme Blend to use, first multiply your collagenase working concentration (in WÃ¼nsch units/ml) times the total volume of your working enzyme solution to obtain the total collagenase activity needed (WÃ¼nsch units). Divide the total collagenase activity required by the Liberase Research Grade stock concentration ( “Reconstitution and Storage”). This indicates how many milliliters of Liberase Research Grade Enzyme Blend stock solution to use in your working enzyme solution.Storage conditions (working solution): Store unused stock solution in single-use aliquots at -15 to -25 °C. For further information on product stability please visit the Roche Liberase Enzyme website at www.collagenase.com.Note: Avoid repeated freezing and thawing!