RPMI-1640 Medium, Hybri-Max, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without s

RPMI-1640 Medium, Hybri-Max, Modified, with L-glutamine, 4500 mg/L glucose and 15mM HEPES, without s

Stock Code: 3586207
Manufacturer Part No: R8005-10X1L
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General description


RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.


Legal Information


Hybri-Max is a trademark of Sigma-Aldrich Co. LLC


Quantity


Formulated to contain 16.4 grams of powder per liter of medium.


Reconstitution


Supplement with 2.0 g/L sodium bicarbonate.

Quality Level500
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - hybridoma: suitable
Storage Temp.2-8°C
Impuritiesendotoxin
Formpowder

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