Iscoves Modified Dulbeccos Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, pow

Descriptions

Application

Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Iscove?s Modified Dulbecco?s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.

General description

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Quantity

Formulated to contain 17.7 grams of powder per liter of medium.

Reconstitution

Supplement with 3.024 g/L sodium bicarbonate.

Specifications

No detailed specifications are available for this product.

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Code	Description	Manufacturer	Technique(s)	Storage Temp.	Form	Price	Quantity
3586147	Iscoves Modified Dulbeccos Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, pow	SIGMA-ALDRICH	cell culture - hybridoma: suitable, cell culture - mammalian: suitable	2-8°C	powder	£146.14 (exc VAT) per 10L	- +
3586148	Iscoves Modified Dulbeccos Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, pow	SIGMA-ALDRICH	cell culture - hybridoma: suitable, cell culture - mammalian: suitable	2-8°C	powder	£172.71 (exc VAT) per EACH	- +
3586149	Iscoves Modified Dulbeccos Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, pow	SIGMA-ALDRICH	cell culture - hybridoma: suitable, cell culture - mammalian: suitable	2-8°C	powder	£591.85 (exc VAT) per 50L	- +