Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N,N-tetraacetic acid tetrasodium salt, >=97%
Application
A chelating agent useful for the determination of calcium in the presence of magnesium. Repeated washing with EGTA-buffer can experimentally deplete cultured cells of Ca2+.
EGTA is suitable for use:in media for chelating Ca2+ to facilitate efficient incorporation of Sr2+ into the oocytesas a component of phosphatase wash buffer used to wash magnetic beads during dephosphorylation and radiolabeling of RNA segments cross-linked to immunoprecipitated proteinsas a component of oocyte activation media
Biochem/physiol Actions
Ethylene glycol-bis(ß-aminoethyl ether)-N,N,N,N-tetra acetic acid tetrasodium salt (EGTA) is a calcium chelator that binds to Ca2+ in the presence of Mg2+ at a ratio of 1:1.
Ethylene glycol-bis(?-aminoethyl ether)-N,N,N?,N?-tetra acetic acid tetrasodium salt (EGTA) dissociates mucin complex and is useful in immunohistochemical studies. It suppresses root growth and mitotic index in germinating wheat seeds. It is used as a decalifying agent in root canal biomechanical preparation and is effective compared to EDTA and cyclohexanediaminetetraacetic acid (CDTA).
Packaging
10, 50 g in glass bottle
| Quality Level | 200 |
|---|---|
| InChI key | BAOGCDPNNOBWQZ-UHFFFAOYSA-J |
| InChI | 1S/C14H24N2O10.4Na/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24;;;;/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);;;;/q;4*+1/p-4 |
| Manufacturer | SIGMA-ALDRICH |
| Solubility | H2O: soluble 10%, clear, colorless |
| Assay | ≥97% |
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