Lambda DNA Hind III Digest, for DNA electrophoresis

Lambda DNA Hind III Digest, for DNA electrophoresis

Stock Code: 3577375
Manufacturer Part No: D9780-.1MG
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Application


Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.


Components


Sigma’;s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.


General description


Sigma’;s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.


Other Notes


Contains 8 fragments, 125–23,130 bp


For optimal resolution, the recommended agarose gel concentration is 0.75%.


Preparation Note


Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Quality Level200, 100
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Suitabilitysuitable for electrophoresis (DNA)
Formliquid

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