Lambda DNA Mixed Digest, for DNA electrophoresis
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Components
Sigma’;s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
General description
Sigma’;s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Other Notes
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Preparation Note
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
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