ROCHE Transforming Growth Factor-beta1 human (hTGF-beta1)recombinant (CHO cells)

Stock Code: 2795595
Manufacturer Part No: 11412272001

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Descriptions

Application

Transforming growth factor-?1, human (hTGF-?1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.

Biochem/physiol Actions

Transforming Growth Factor-?1, human (hTGF-?1), is a multifunctional growth factor affecting many functions in a variety of different cells.

Transforming growth factor-? (TGF-?) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.

General description

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.Solution in PBS and 1 mg/ml BSA, filtered through 0.2 µm pore size membrane.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 0.01 - 10 ng/mlRecombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.Working solution: Dilute the concentrated TGF-β1 solution (1 µg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.Note: Avoid repeated freezing and thawing.

Quality

Purity: >95% (SDS-PAGE)Endotoxin level: ﹤0.1 EU/µg (LAL-test), ﹤10 EU/ml (LAL-test)(Note: 1 EU corresponds to 0.1 ng)

Sequence

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Chain Length 2 x 112 AA

Specificity

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.Specific activity/EC₅₀: ﹤0.1 ng/ml, at least the same specific activity (EC﹤SUB>50) compared to the indicated standard is guaranteed.

Unit Definition

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.