BM Condimed H1 (Hyb.-Cloning-Suppl.)

Stock Code: 2795527
Manufacturer Part No: 11088947001
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£649.07 (exc VAT) per EACH
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Analysis Note


Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.


Application


BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.


Cell Line Origin


BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.


Features and Benefits


ContentsSolution in RPMI 1640, filtered through 0.2µm pore size membrane


General description


BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.


Other Notes


For life science research only. Not for use in diagnostic procedures.


Physical form


Solution in RPMI 1640, filtered through 0.2 µm pore size membrane


Preparation Note


Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.

Quality Level100
Technique(s)cell culture - hybridoma: suitable
Storage Temp.−20°C
Solubilitywater: miscible
Formsolution

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