Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, >=98.5% (GC

Stock Code: 3577432
Manufacturer Part No: L3771-1KG
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Analysis Note


Tested for use in denatured polyacrylamide gel electrophoresis.


Application


Sodium dodecyl sulfate has been used: in chromatin immunoprecipitation in SDS-polyacrylamide gel electrophoresis (SDS-PAGE) as a component of radioimmunoprecipitation assay buffer for immunoblotting


Used to solubilize and denature proteins for denaturing-PAGE. Most proteins bind SDS in a ratio of 1.4 g SDS per gram of protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge to mass ratio is essentially the same for each protein and will migrate in the gel based only on their size.


Anionic detergent


General description


Sodium dodecyl sulfate (SDS) is an anionic detergent suitable to denature proteins. It has a long-chain aliphatic group with negatively charged sulfate attached. This makes SDS an amphipathic detergent.


Packaging


25, 100, 500 g in poly bottle


1 kg in poly bottle

Quality Level200
Pack1KG
mp204-207 °C (lit.)
InChI keyDBMJMQXJHONAFJ-UHFFFAOYSA-M
InChI1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
ManufacturerSIGMA-ALDRICH
Further Infoanionic
Product LineBioReagent
Suitabilitysuitable for electrophoresis
Solubilitywater: soluble
Assay≥98.5% (GC)

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