cOmpleteEDTA-freeEASYpack20 Tab.

Stock Code: 2795268
Manufacturer Part No: 4693132001

Order Now for 21 day delivery

Descriptions

Application

cOmplete^™, EDTA-free Protease Inhibitor Cocktail has been used as a component in lysis buffer for the lysis of transfected human embryonic kidney (HEK293T) cells and in radioimmunoprecipitation assay (RIPA) lysis buffer for the homogenization of zebrafish embryo tissue protein extraction reagent (T-PER) for the homogenization of hippocampus tissue sodium dodecyl sulphate (SDS) lysis buffer for the lysis of hepatocytes

cOmplete^™, EDTA-free, efficiently inhibits serine and cysteine proteases in bacterial, yeast, plant, and animal cell extracts. Metallo- and aspartic proteases are not inhibited.cOmplete, EDTA-free Tablets, are used for the inhibition of proteolytic activity in large volumes (up to 50ml) in which EDTA may interfere with protein stability (e.g., metal-containing proteins) or subsequent assays. Since EDTA interferes with IMAC (Immobilized Metal Affinity Chromatography), cOmplete, EDTA-free is preferentially used in the isolation process of Poly-His-tagged fusion proteins.If it is necessary to inhibit proteolytic activity in a smaller volume (up to 10ml), we recommend to use cOmplete, Mini, EDTA-free.One cOmplete, EDTA-free tablet was added per 50 ml incubation solution. Proteolytic activity was determined with the Roche Universal Protease Substrate (casein, resorufin-labeled). When extractions or single-step isolations are necessary in the acid pH range, simply include pepstatin along with cOmplete, EDTA-free tablets to ensure aspartic (acid) protease inhibition. All experiments were performed at +15 to +25°C.

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Features and Benefits

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results. Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer. Complete protection: Instantly protect your proteins against a broad range of proteases. Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. Safe: Choose non-toxic inhibitors that pose no risk to you or those around you. Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

General description

cOmplete, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other cOmplete tablets they do not contain EDTA - no other chelating agents, e.g. EGTA as well - leaving the stability and the function of metal-dependant proteins unaffected. The affinity purification of Poly-His-tagged fusion proteins using IMAC (Immobilized Metal Affinity Chromatography) is also facilitated (no dialysis necessary)