ROCHE Pwo Master

ROCHE Pwo Master

Stock Code: 2795254
Manufacturer Part No: 3789403001
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£303.64 (exc VAT) per EACH
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Application


Use this premixed, double-concentrated formulation containing PwoSuperYield DNA Polymerase, a new optimized buffer system, and PCR grade nucleotides, if your application requires better fidelity than provided by (FastStart) Taq DNA Polymerase. The product can be applied for: Accurate amplification of DNA critical applications High-throughput PCR High fidelity PCR Site-directed mutagenesis Cloning Gene expression


Features and Benefits


Pwo Master is a ready-to-use, 2x concentrated master mix. Pwo SuperYield DNA Polymerase has polymerase and 3′ → 5′ exonuclease proofreading activity, including an optimized buffer. The buffer enhances enzyme performance, synthesizing higher DNA yields of high fidelity. Higher yield and 18-fold higher fidelitycompared to Taq DNA Polymerase. Maximize convenience.Fewer steps are required with the ready-to-use master mix. Simply add template DNA, primers, and PCR-grade water then start the reaction. Reduce setup time.Store at +2 to +8°C - no need to thaw reagents.


General description


Pwo SuperYield DNA Polymerase is originally isolated from Pyrococcus woesei.The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.PCR products are blunt-ended directly useable for blunt-end ligation.Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.The core component of the Pwo Master is the proofreading Pwo SuperYield DNA Polymerase which has both polymerase activity and a 3′–5′ exonuclease (proofreading) activity. Pwo SuperYield DNA Polymerase combines Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the enzyme, resulting in higher yields of the amplification without changing the fidelity of DNA synthesis.


Legal Information


Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.﹤/ br>NOTICE TO PURCHASER: LIMITED LICENSE﹤br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research

Quality Level100
Parameter72 °C optimum reaction temp.
Storage Temp.2-8°C

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