DL-Dithiothreitol, for electrophoresis, >=99%

DL-Dithiothreitol, for electrophoresis, >=99%

Stock Code: 3577421
Manufacturer Part No: D9163-5G
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£262.50 (exc VAT) per 5G
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Analysis Note


Tested for use in reduced SDS-PAGE


Application


An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).


Packaging


1, 5, 25 g in poly bottle

Quality Level200
Pack5G
mp40-44 °C, 41-44 °C (lit.)
InChI keyVHJLVAABSRFDPM-QWWZWVQMSA-N
InChI1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
ManufacturerSIGMA-ALDRICH
Formpowder
Assay≥99%

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