CHAPS hydrate, BioReagent, suitable for electrophoresis, >=98% (TLC)
Application
CHAPS has been used:For membrane permeabilization of cellsAs a component in lysis buffer for homogenization of liver tissues as well as adipose tissueAs a component in denaturing buffer in enzyme characterizationAs a component of 2D- sample buffer in 2D electrophoresisFor protein solubilization in isoelectric focusing
General description
CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.
Packaging
1, 5 g in glass bottle
| Pack | 1G |
|---|---|
| mp | 157 °C (dec.) (lit.) |
| InChI key | SJCUTFKCLFLIFE-JWTJKVBLSA-N |
| InChI | 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30 |
| Manufacturer | SIGMA-ALDRICH |
| Further Info | zwitterionic |
| Impurities | ﹤0.04% DMF |
| Form | powder |
| Assay | ≥98% (HPLC) |
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