Bluo-Gal, reagent for selection of recombinant bacterial clones

Bluo-Gal, reagent for selection of recombinant bacterial clones

Stock Code: 3577193
Manufacturer Part No: B2904-500MG
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£618.43 (exc VAT) per 500MG
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Application


Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.


Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as ?-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.


General description


Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.


Packaging


100, 500 mg in poly bottle


Principle


When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Quality Level200
InChI keyLINMATFDVHBYOS-MBJXGIAVSA-N
InChI1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1
ManufacturerSIGMA-ALDRICH
SolubilityDMF: soluble
Formpowder
Assay≥98% (HPLC)

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